The CFX96 Touch Amplifier (Bio-Rad, USA) is designed for conducting PCR with real-time registration of reaction products. It features a six-channel detection system that provides high speed and flexibility for PCR diagnostics.
Real-time PCR offers the following advantages:
• Quantitative assessment of genetic material from multiple infectious agents in dozens of samples.
• Prevention of contamination - all stages of analysis in one tube.
• Automatic registration of reaction product concentrations directly during amplification.
• Highest sensitivity, versatility, and specificity.
• Ability to place the genetic diagnostics laboratory on one table.
• Reduction of analysis time.
Applications:
• Real-time PCR (up to 5 dyes in one tube).
• PCR with endpoint analysis.
• PCR without result analysis.
• Analysis of melting curves, including HRM.
• FRET analysis.
• Selection of optimal PCR temperature.
Technical specifications:
• Open system: ability to use test systems from various manufacturers
• Compatibility with robotic sample preparation stations is ensured by automatic loading of the device
• CFX Manager ™ software can send notifications of reaction completion with an attached file of obtained data to your e-mail
• Block format: 96 samples / 384 samples
• Tube type: 96-well plate or 0.2 ml tubes (separately or in strips) / 384-well plate
• Number of channels: 6
• Reaction mixture volume 10-50 µl
• Maximum heating / cooling rate: 5 °C / sec
• Temperature range: 0-100 °C; temperature accuracy: ± 0.2 °C; temperature uniformity: ± 0.4 °C after 10 sec at 90 °C
• Average speed: 3.3 °C / sec
• Gradient range: 30-100 °C
• Programmable heating / cooling of the reaction module with Peltier elements: yes
• Type of fluorescence detection: 6 highly sensitive photodiodes with filters
• Type of fluorescence excitation: 6 LEDs with filters
• Fluorescence excitation range: 450-684 nm
• Fluorescence detection range: 515-730 nm
• FRET registration: yes
• Dimensions (L.W.H): 13.18.14 cm
• Weight: 21.4 kg
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